TC-CPE “hijacks” WT CPE in N2A cells.

<p><b>(A)</b> Representative Western blot analysis of CPE expression from cell lysates after various treatments for 24 h in N2A cells. <b>(B)</b> Bar graphs show the quantification of CPE expression from cell lysates normalized to actin. <b>(C)</b> Representative Western blot analysis of secreted CPE after various treatments for 24 h in N2A cells. <b>(D)</b> Bar graphs show the quantification of secreted CPE levels. For CPE secretion, one-way ANOVA (F_(3,8) = 164.9, p<0.001, n = 3) followed by Tukey’s multiple comparison test. <b>(E)</b> N2A cells were transfected with EV, WT-CPE or WT-CPE +TC-CPE for 24 h, and then 100 μM H₂O₂ was added for 48 h. LDH release assay indicated that the neuroprotection of WT-CPE against oxidative stress was not affected by co-transfection with TC-CPE in N2A cells. One-way ANOVA analysis followed by Tukey’s multiple comparison test: F_(2,12) = 7.139, p < 0.05, n = 5.